Biochemistry

   

The Influence of Biofield Energy Treated Vitamin D3 on Bone Health and Its Health Consequences in MG-63 Cell-Line

Authors: Elizabeth Patric, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana

The aim of this study was to assess the effect of Consciousness Energy Healing-based vitamin D3 and DMEM medium on bone health parameters such as alkaline phosphatase (ALP), collagen, and bone mineralization in human bone osteosarcoma cells (MG-63). The test items (vitamin D3 and DMEM), were divided into two parts. One part of each sample was received the Biofield Energy Treatment by Elizabeth Patric and those samples were denoted as the Biofield Energy Treated (BT) samples, while the other parts of each sample were referred as the untreated test items (UT). The MTT cell viability assay revealed that the test samples were found as safe in the tested concentrations. The experimental results showed that the level of ALP was remarkably increased by 88.62% and 70.48% in the BT-DMEM + UT-Test item (UT-TI) and BT-DMEM + BT-TI groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-TI group. Moreover, the collagen level was significantly increased by 252.59%, 50.55%, and 94.99% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-TI group. Further, the collagen level was significantly increased by 120.90% and 72.66% in the UT-DMEM + BT-TI and BT-DMEM + UT-TI groups, respectively at 50 µg/mL, while 34.99% in the UT-DMEM + BT-TI at 100 µg/mL compared to the untreated group. Apart from this, the percent of bone mineralization was distinctly enhanced by 127.13%, 225.12%, and 106.28% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 0.1 µg/mL compared to the untreated group. Moreover, the percentage of bone mineralization was significantly increased by 52.86%, 67.00%, and 23.62% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 1 µg/mL compared to the untreated group. Additionally, the percentage of bone mineralization was significantly increased by 46.57% and 51.36% in the UT-DMEM + BT-TI and BT-DMEM + UT-TI groups, respectively at 10 µg/mL compared to the untreated group. Altogether, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be an alternative approach for nutraceutical supplement to combat vitamin D3 deficiency and able to fight against various bone-related disorders including rickets, low bone density, osteomalacia, bone and joint pain, bone fractures, osteoporosis, osteoma, osteogenesis imperfecta, Paget’s disease, deformed bones, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.

Comments: 9 Pages.

Download: PDF

Submission history

[v1] 2018-07-26 02:19:24

Unique-IP document downloads: 74 times

Vixra.org is a pre-print repository rather than a journal. Articles hosted may not yet have been verified by peer-review and should be treated as preliminary. In particular, anything that appears to include financial or legal advice or proposed medical treatments should be treated with due caution. Vixra.org will not be responsible for any consequences of actions that result from any form of use of any documents on this website.

Add your own feedback and questions here:
You are equally welcome to be positive or negative about any paper but please be polite. If you are being critical you must mention at least one specific error, otherwise your comment will be deleted as unhelpful.

comments powered by Disqus